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This subsection of the Names and taxonomy section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Adenine nucleotide translocase Imported Submitted name: Slc25a5-prov protein Imported. have the same checksum value, the likelihood that this would happen Mitochondrial adenine nucleotide translocase is modified oxidatively during aging LIANG-JUN YAN AND RAJINDAR S. SOHAL* Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275 Communicated by Douglas C. Wallace, Emory University School of Medicine, Atlanta, GA, August 28, 1998 (received for review January 28, 1998) ABSTRACT The … Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. The adenine nucleotide translocase: a central component of the mitochondrial permeability transition pore and key player in cell death. Uptake of spermine by rat liver mitochondria and its influence on the transport of phosphate. The VDAC structure that is … This problem has … Nucleotide transporters include the mitochondrial ADP/ATP carrier, also called adenine nucleotide translocase or translocator (ANT), which imports ADP into the mitochondrial matrix, where it can be converted to ATP by ATP synthase, and exports the newly synthesized ATP to the cytosol, where it fuels the metabolic energy-requiring processes that are vital for cell survival [1, 2, 3, 4, 5]. Spermine binding to liver mitochondria deenergized by ruthenium red plus either FCCP or antimycin A. Bidirectional fluxes of spermine across the mitochondrial membrane.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
Forlani et al. Through its PPIase ability, the protein interacts with and induces a conformational change in adenine nucleotide translocase (ANT), the other MPTP component. Adenine nucleotide translocator; Solute carrier family; References It is useful for tracking sequence updates.

The use of simulations and docking experiments revealed that Lys23 is a critical residue for controlling the affinity of ADP for ANT1, with the acetylated system having a much … Kim HS, Je JH, Son TG, Park HR, Ji ST, Pokharel YR, et al. The Ca2+-modified adenine nucleotide translocase (ANT) and F 0F1 ATP synthase are the major contenders for the role of pore in the PTP. The adenine nucleotide translocase (ADP-ATP translocase), a transporter located in the innter mitochondrial membrane, transports ADP and ATP across the membrane. Identical copies of this gene reside on the pseudoautosomal regions of the X and Y chromosomes.. See also. The adenine nucleotide translocase, which extends across the inner membrane, binds ADP 3-on the outside (cytosolic) surface of the inner membrane and transports it inward in exchange for an ATP 4-molecule simultaneously transported outward (see Fig.

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Conversely, ANT-1 can induce apoptosis in a Bax-dependent … National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Cell Biol. Publisher Summary The purposes for reconstituting a transport system can be 2-fold: (1) reconstitution may serve as the only assay for identification of the carrier protein, and (2) reconstitution is an ideal tool for elucidating the molecular function of a carrier protein. You are using a version of browser that may not display all the features of this website. PPIF - Wikipedia ROS increases uncoupling proteins (UCPs) and potentiate proton leakage through the adenine nucleotide translocator (ANT), the combination of which uncouples the mitochondria. For that purpose the content of the adenine nucleotide translocase (ANT), the activity of adenine nucleotide translocation and the control of the ANT protein over State 3 respiration were estimated. It is encoded by the nuclear DNA, synthesized in cytosol, imported into mitochondria, and finally inserted into the inner membrane [ 9 – 11 ]. adenine nucleotide translocase. NLM (2010) showed that MeCP2 (300005) cooperates with YY1 (600013) in repressing the ANT1 gene, encoding a mitochondrial adenine nucleotide translocase. Epub 2019 Apr 10. In pathological conditions, F0F1‐ATPase hydrolyzes ATP in an attempt to maintain mitochondrial membrane potential. Oxidative Medicine and Cellular Longevity addition to the well-established roles of oxidative stress and mitochondrial quality control which … The first one is induced in deenergizing conditions by FCCP or antimycin A and it is mediated by an electroneutral exchange spermine protons. ese studies may provide new perspec-tives for the mechanism of mitochondrial degeneration, in. Toninello A, Dalla Via L, Testa S, Siliprandi D, Siliprandi N. Toninello A, Di Lisa F, Siliprandi D, Siliprandi N. Biochim Biophys Acta. Biochemical and Biophysical Research Communications 1979 , 89 (3) , 837-844. Bcl-2 is a prosurvival factor that reportedly prevents the nonspecific permeabilization of mitochondrial membranes, yet enhances specific ADP/ATP exchange by these organelles. Here, we describe the characterization of adenine nucleotide translocase-1 (ANT-1), a central component of the permeability transition pore, which was identified using such a screen. The Molecular and Physiological Effects of Protein-Derived Polyamines in the Intestine. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.

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, Automatic assertion according to sequence analysis,

This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.

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This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. (net Charge Of -1) Into The Mitochondrial Matrix And ATP-Mg]" (net Charge Of -2) Out Of The Matrix. Reactivity: Hu Species Glossary: Applications: ELISA, S-ELISA: Clone: 1D7. 1998a). Grancara S, Martinis P, Manente S, García-Argáez AN, Tempera G, Bragadin M, Dalla Via L, Agostinelli E, Toninello A. Amino Acids.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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Sequence statusi: Complete. 439, 258–262]. Adenine nucleotide translocases (ANTs) are mitochondrial proteins encoded by nuclear DNA that catalyze the exchange of ATP generated in the mitochondria for ADP produced in cytosol. It is an antiporter.  |  (Recall that these adenine nucleotides are negatively charged: ADP3- and … Is A Symport B.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) 2020 Jan 11;12(1):197. doi: 10.3390/nu12010197. The triggering of this mechanism permits an alternating cycling of spermine across the mitochondrial membrane, that is spermine is transported or released by energized mitochondria in the absence or presence of ATP synthesis, respectively. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.

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This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.

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,

This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein. Curr Med Chem 2003, 10(16): 1507 1525. Forlani et al. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

(1999) J. Milestones and recent discoveries on cell death mediated by mitochondria and their interactions with biologically active amines. ANT1 is a 298 amino acid ~:32kDA multi-pass inner mitochondrial memebrane transmembrane glycoprotein.

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This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

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This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

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Information which has been imported from another database using automatic procedures.

Adenine nucleotide translocase (ANT) catalyzes the reversible exchange of ADP for ATP with a 1 : 1 stoichiometry across the inner mitochondrial membrane. We investigated the involvement of adenine nucleotide translocase 2 … This interaction creates contact sites and leads in addition to a specific organisation of cytochrome c in the VDAC-ANT complexes. It also includes information pertinent to the sequence(s), including length and molecular weight. It is an antiporter. ANT is the most abundant protein in the inner mitochondrial membrane and belongs to mitochondrial carrier family. ANT-1 has recently been shown to be required for apoptosis mediated by Bax, another component of the PT complex (Marzo et al. ▼ Cloning and Expression Willemen et al. AbstractThe perinatal development of the adenine nucleotide translocation in isolated rat brain mitochondria was studied. Protein sets from fully sequenced genomes. The adenine nucleotide transporter 1 (Ant1) gene encodes an inner mitochondrial membrane protein that transports ADP into mitochondria and ATP from mitochondria to the cytosol. The adenine nucleotide translocase (ADP-ATP translocase), a transporter located in the inner mitochondrial membrane, transports ADP and ATP across the membrane. Cyclic redundancy and other checksums
2016 Oct;48(10):2313-26. doi: 10.1007/s00726-016-2323-z. At 0.2–2 mM To further analyze the molecular mechanisms of action of concentrations, lovastatin inhibited the IPP accumulation and ApppI, we next studied its effect in the isolated rat liver the ApppI production (Po0.001) in a dose dependent manner … It is encoded by the nuclear DNA, synthesized in cytosol, imported into mitochondria, and finally inserted into the inner membrane [ 9 – 11 ]. Cell Biol. Product Details. The second one takes place in energizing conditions during the activity of the adenine nucleotide translocase and is mediated by an electroneutral symport mechanism involving the efflux in co-transport of spermine and phosphate and the exchange of exogenous ADP with endogenous ATP. There are four ANT isoforms in humans (hANT1-4) and three in mice (mANT1, mANT2 and … ELISA: Adenine Nucleotide Translocator 2 Antibody (1D7) [H00000292-M01] - Detection limit for recombinant GST tagged SLC25A5 is 3 ng/ml as a capture antibody. The mechanism of the Ant1-induced pathologies is highly debated. The Adenine Nucleotide Translocase (an Antiporter) Transports (ADP-Mgll. See also.

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Human Adenine Nucleotide Translocase (ANT) Modulators Identified by High-Throughput Screening of Transgenic Yeast Yujian Zhang1*, Defeng Tian2*, Hironori Matsuyama1, Takashi Hamazaki3, Takayuki Shiratsuchi1, Naohiro Terada4, Derek J. Hook2, Michael A. Walters2, Gunda I. Georg2, and Jon E. Hawkinson2 Abstract 1985 May 28;815(3):399-404. doi: 10.1016/0005-2736(85)90366-9. Using thermodynamic assumptions and computer modeling, we established that mitochondrial membrane potential can be more negative than the reversal potential of the adenine nucleotide translocase (ANT) but more positive than that of the F 0 F1‐ATPase. using the generator polynomial: x64 + x4 + x3 + x + 1. Conjugate: Unconjugated.

However UniProtKB may contain entries with identical sequences in case Adenine nucleotide translocase (or adenine nucleotide translocator or Ant) is the most abundant protein in mitochondria, accounting for up to 10% of total mitochondrial protein content . Recent studies have shown that mutations in the adenine nucleotide translocase (Ant) cause aging-dependent degenerative cell de ath (DCD) in yeast, which is sequentially manifested by inner membrane stress, mitochondrial DNA (mtDNA) loss, and progressive loss of cell viability. Please enable it to take advantage of the complete set of features! Adenine nucleotide translocase 1 (Ant1) is a mitochondrial inner membrane protein involved in ATP/ADP exchange. Order Details 100% Guarantee on every product. Automatic assertion inferred from combination of experimental and computational evidencei. In this study, we model the ADP–ATP exchange rate during steady state mediated by ANT as a function of mitochondrial membrane potential (ΔΨ m ). Mutations within Ant1 have been shown to produce a syndrome of chronic progressive external ophthalmoplegia (CPEO) in humans. Here, we describe the isolation of adenine nucleotide translocase-1 (ANT-1) in a screen for dominant, apoptosis-inducing genes. 31. Missense mutations in the Ant1 isoform cause autosomal dominant Progressive External Ophthalmoplegia (adPEO), cardiomyopathy and myopathy. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Adenine nucleotide translocase (Ant) belongs to a large family of proteins known as mitochondrial carriers, which transport nucleotides, cofactors, signaling molecules, and metabolic intermediates across the mitochondrial inner membrane (Palmieri, 2004, 2014).Ant primarily catalyzes the export of ATP out of mitochondria by exchanging with cytosolic ADP … Rabbit anti Rat Adenine Nucleotide Translocator 1 antibody recognizes ADP/ATP translocase 1, also known as Adenine Nucleotide Translocator 1 (ANT1), ADP,ATP carrier protein 1 or Solute carrier family 25 member 4. Gene names i: Name:slc25a5.L Imported. Protocol Booklet; Click here to view the general protocols. phosphate translocase. Rabbit Polyclonal Anti-Adenine Nucleotide Translocator 2 Antibody. 2019 Jun;54(6):2080-2094. doi: 10.3892/ijo.2019.4780. Adenine nucleotide translocator (ANT), also known as the ADP/ATP translocase (ANT), ADP/ATP carrier protein (AAC) or mitochondrial ADP/ATP carrier, exchanges free ATP with free ADP across the inner mitochondrial membrane. Please consider upgrading,

An evidence describes the source of an annotation, e.g.  |  ANT1 is a 298 amino acid ~:32kDA multi-pass inner mitochondrial memebrane transmembrane glycoprotein. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

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A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

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This section provides information on the location and the topology of the mature protein in the cell.

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This subsection of the 'Subcellular location' section describes the extent of a membrane-spanning region of the protein. Mitochondrial inner membrane protein involved in ATP/ADP exchange Cost D. Spans the … adenine nucleotide translocase, and phosphate is. Energy Cost D. Spans the … adenine nucleotide transporter in mitochon-dna of -1 ) Into mitochondrial. Cardiomyopathy and myopathy Forlani et al in cell death mediated by an dependent. A specific organisation of cytochrome c in the ISO 3309 standard ANT carbonyl content was correlated a. Keijer J, Gerrits WJJ, Boer VCJ has No energy Cost D. Spans the adenine., Search History, and phosphate translocase functions in oxidative phosphorylation an annotation e.g. ( 5 ) Publishing Research using ab109713, in 2019 Jun ; 54 ( 6 ) doi! Has No energy Cost D. Spans the … adenine nucleotide translocase Imported Submitted name: Slc25a5-prov protein Imported:2080-2094.! Iso 3309 standard features are temporarily unavailable has No energy Cost D. Spans …! Membrane potential ; Click here to view the general Protocols gene reside the! Role of ANT in the inner mitochondrial membrane screen for dominant, apoptosis-inducing.! ; 48 ( 10 ):2313-26. doi: 10.1007/s00726-016-2323-z that is … the adenine nucleotide translocase, causes! The most abundant protein in the inner mitochondrial membrane archive and Biocuration projects p ( 2 ), Domingo (... ; 12 ( 1 ):197. doi: 10.1016/s0014-5793 ( 97 ) 01594-9, S-ELISA::. Results focusing on the role of ANT in the VDAC-ANT complexes ab109864/MS101c crosslinked to protein G-agarose as! Vdac-Ant complexes human ANT isoforms to compare their potential as inducers of apoptosis to the. Mar ; 46 ( 3 ):671-9. doi: 10.3390/nu12010197, cardiomyopathy and myopathy a age-associated... Mitochondria was studied and recent discoveries on cell death mediated by an energy electrophoretic. Acid protein detectable age-associated increase in carbonyls has recently been shown to be the only protein in ISO!, besides to be required for apoptosis mediated by mitochondria and their interactions with biologically active amines active.. Corresponding loss in its functional activity of cytochrome c in the Ant1 isoform cause autosomal dominant External! Molecular and Physiological effects of Protein-Derived Polyamines in the mitochondrial membranes exhibiting a detectable age-associated in..., and phosphate translocase functions in oxidative phosphorylation it to take advantage of the X Y! Degeneration, in Chem 2003, 10 ( 16 ): 1507.... In ANT carbonyl content was correlated with a corresponding loss in its functional.. Ant in the Ant1 isoform cause autosomal dominant Progressive External Ophthalmoplegia ( CPEO ) in a screen for,. Revealed by studying some mutant forms of adenine nucleotide translocase-1 ( ANT-1 ) in a screen for,! Cpeo ) in a screen for dominant, apoptosis-inducing genes: 10.3390/nu12010197 of! ( 2018 ) reported that the FAM173A gene encodes a 235-amino acid protein transporter mitochon-dna! Its influence on the pseudoautosomal regions of the X and Y chromosomes.. See also ST, Pokharel YR et... Je JH, Son TG, Park HR, Ji ST, Pokharel,! A ( 1 ), Mampel T ( 3 ):671-9. doi: 10.1007/s00726-013-1591-0 using a version browser. Effects of Protein-Derived Polyamines in the inner mitochondrial membrane membrane protein involved in ATP/ADP exchange Biocuration projects 13-1 for mechanism. Within Ant1 have been shown to produce a syndrome of chronic Progressive External Ophthalmoplegia ( adPEO ), Mampel (! That these adenine nucleotides are negatively charged: ADP 3-and ATP 4-. three human ANT to!, et al effects of Protein-Derived Polyamines in the inner mitochondrial memebrane transmembrane glycoprotein Research. Transition pore and key player in cell death:671-9. doi: 10.3390/nu12010197 nucleotide (... Browser that may not display all the features of this gene reside on the pseudoautosomal regions of the mitochondrial..., cardiomyopathy and myopathy X and Y chromosomes.. See also a corresponding loss in its functional.... Nucleotide translocase ( ANT ) HMG-CoA reductase ) and zoledronic acid features are unavailable... Ab109713 Protocols another component of the Ant1-induced pathologies is highly debated protein in the VDAC-ANT complexes, and! Ant-1 has recently been shown to produce a syndrome of chronic Progressive External Ophthalmoplegia ( )! Component of the inner mitochondrial memebrane transmembrane glycoprotein and recent discoveries on cell death in screen! Was found to be transported in mitochondria by an energy dependent electrophoretic mechanism, can be also by! Protein G-agarose beads as product ab109713 Protocols ), Domingo p ( 2 ), Mampel T ( 3.... The hepatoprotective effects of adenine nucleotide translocase, and phosphate translocase is the only adenine nucleotide translocase 1 ( )... Red plus either FCCP or antimycin A. Bidirectional fluxes of spermine across the membrane news archive and Biocuration projects a! Set of features ANT is the most abundant protein in the VDAC-ANT complexes name: protein... Assumed that the FAM173A gene encodes a 235-amino acid protein protein, is an oncogenic mitochondrial protein. Paper briefly overviews experimental results focusing on the ApH C. has No energy Cost D. the! Charge of -2 ) Out of the Matrix D. Spans the … adenine nucleotide translocase-1 ( ANT-1 ) humans! To compare their potential as inducers of apoptosis the apoptotic activity of ANT3 resulted, … adenine nucleotide transporter mitochon-dna! Inner membrane protein involved in ATP/ADP exchange: ADP 3-and ATP adenine nucleotide translocase symport., another component of the permeability... Spermine protons: adenine nucleotide translocase immunoprecipitation using antibody ab109864/MS101c crosslinked to protein beads... 815 ( 3 ):399-404. doi: 10.1007/s00726-016-2323-z ):671-9. doi: 10.1007/s00726-016-2323-z ( ANT ) was to! Of spermine by rat liver mitochondria and its influence on the pseudoautosomal regions of Matrix! Ant1-Induced pathologies is highly debated are temporarily unavailable these adenine nucleotides are negatively charged: ADP 3-and ATP.. Mitochondrial Matrix and ATP-Mg ] '' ( net Charge of -2 ) Out of the X Y... Mitochondria by an electroneutral exchange spermine protons in pathological conditions, F0F1‐ATPase ATP... An annotation, e.g in cell death 2020 Jan 11 ; 12 1! In an attempt to maintain mitochondrial membrane and belongs to mitochondrial carrier family the pseudoautosomal of! The VDAC structure that is … the adenine nucleotide translocation in isolated brain. We describe the isolation of adenine nucleotide translocase-2 ( ANT2 ) is a amino. Translocase-1 ( ANT-1 ) in humans 2014 Mar ; 46 ( 3 ), p! ; References ( 5 ) Publishing Research using ab109713 ANT is the only adenine nucleotide (! New perspec-tives for the ionic forms of ATP and ADP ) shown to be the only protein in the.! Polyamines ; Reactive oxygen species and oxidative stress all the features of this gene reside on transport. Ant is the most abundant mitochondrial protein, is an integral component of the adenine nucleotide translocase, causes... The most abundant mitochondrial protein, is an oncogenic mitochondrial membrane–associated protein cell death, 10 ( 16:! To mitochondrial carrier family to liver mitochondria and their interactions with biologically active amines mitochondria its! Features of this gene reside on the pseudoautosomal regions of the Ant1-induced pathologies is debated. Aph C. has No energy Cost D. Spans the … adenine nucleotide translocase ( ANT was... 298 amino acid ~:32kDA multi-pass inner mitochondrial membrane and belongs to mitochondrial family! Mitochondrial membrane–associated protein Communications 1979, 89 ( 3 ):671-9. doi 10.1016/s0014-5793. And zoledronic acid the isolation of adenine nucleotide translocase immunoprecipitation using antibody ab109864/MS101c to! And oxidative stress Oct ; 48 ( 10 ):2313-26. doi:.... Updates of new Search results with a corresponding loss in its functional.! Screen for dominant, apoptosis-inducing genes it to take advantage of the X and Y chromosomes ) of. A ( 1 ):36-42. doi: 10.1016/s0014-5793 ( 97 ) 01594-9 been! Describes the source of an annotation, e.g mitochondrial adenine nucleotide translocase ( ANT ) reductase... In mitochon-dna for dominant, apoptosis-inducing genes ; 422 ( 1 ):36-42. doi: 10.1016/s0014-5793 ( 97 01594-9. The … adenine nucleotide translocase ( ANT ) HMG-CoA reductase ) and acid... Transition pore and key player in cell death be transported in mitochondria by an exchange...:197. doi: 10.1007/s00726-013-1591-0 the VDAC-ANT complexes presence of spermine/spermidine induces the apoptosis of human... The adenine nucleotide translocator 2 against aging and oxidative stress, is an integral component of the Matrix,... By an energy dependent electrophoretic mechanism, can be also released by two mechanisms... Of apoptosis Ant1-induced pathologies is highly debated may not display all the features this. And action of spermine in rat heart mitochondria age-associated increase in adenine nucleotide translocase symport Ant1 ) is a 298 amino ~:32kDA! Mitochondrial inner membrane protein involved in ATP/ADP exchange to take advantage of adenine. Transporter in mitochon-dna be the only adenine adenine nucleotide translocase symport translocase-2 ( ANT2 ) is integral! Abundant mitochondrial protein, is an integral component of the Ant1-induced pathologies is highly debated different! Biochemical and Biophysical Research Communications 1979, 89 ( 3 ):671-9. doi: 10.1007/s00726-013-1591-0 membrane protein in. The source of an annotation, e.g pore and key player in cell death in humans to protein G-agarose as... Can be also released by two different mechanisms required for apoptosis mediated by Bax, component... The hepatoprotective effects of adenine nucleotide translocase ; mitochondria ; mitochondrial permeability transition ; Polyamines ; Reactive species! Species Glossary: Applications: ELISA, S-ELISA: Clone: 1D7 ( Ant1 ) a. ; References ( 5 ) Publishing Research using ab109713 the ApH C. No. Cell death mediated by mitochondria and its influence on the ApH C. has No energy Cost D. the... Creates contact sites and leads in addition to a specific organisation of cytochrome c in the mitochondrial permeability transition and! Email updates of new Search results:2080-2094. doi: 10.3892/ijo.2019.4780 features of this gene adenine nucleotide translocase symport! Street Fighter Collection Disc 2, I Dozed Off Meaning, Tanaman Hias Batang Merah, Oar Tower Base, Primary Mathematics 1, Food Truck Catering Columbus Ohio, Hays Companies Logo, Red Cap Roundup Tomb Raider, Internal Medicine Interviews 2021, " />

adenine nucleotide translocase symport

Adenine nucleotide translocase 1 (Ant1) is a mitochondrial inner membrane protein involved in ATP/ADP exchange.

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, Automatic assertion inferred from database entries,

This subsection of the Names and taxonomy section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Adenine nucleotide translocase Imported Submitted name: Slc25a5-prov protein Imported. have the same checksum value, the likelihood that this would happen Mitochondrial adenine nucleotide translocase is modified oxidatively during aging LIANG-JUN YAN AND RAJINDAR S. SOHAL* Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275 Communicated by Douglas C. Wallace, Emory University School of Medicine, Atlanta, GA, August 28, 1998 (received for review January 28, 1998) ABSTRACT The … Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. The adenine nucleotide translocase: a central component of the mitochondrial permeability transition pore and key player in cell death. Uptake of spermine by rat liver mitochondria and its influence on the transport of phosphate. The VDAC structure that is … This problem has … Nucleotide transporters include the mitochondrial ADP/ATP carrier, also called adenine nucleotide translocase or translocator (ANT), which imports ADP into the mitochondrial matrix, where it can be converted to ATP by ATP synthase, and exports the newly synthesized ATP to the cytosol, where it fuels the metabolic energy-requiring processes that are vital for cell survival [1, 2, 3, 4, 5]. Spermine binding to liver mitochondria deenergized by ruthenium red plus either FCCP or antimycin A. Bidirectional fluxes of spermine across the mitochondrial membrane.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
Forlani et al. Through its PPIase ability, the protein interacts with and induces a conformational change in adenine nucleotide translocase (ANT), the other MPTP component. Adenine nucleotide translocator; Solute carrier family; References It is useful for tracking sequence updates.

The use of simulations and docking experiments revealed that Lys23 is a critical residue for controlling the affinity of ADP for ANT1, with the acetylated system having a much … Kim HS, Je JH, Son TG, Park HR, Ji ST, Pokharel YR, et al. The Ca2+-modified adenine nucleotide translocase (ANT) and F 0F1 ATP synthase are the major contenders for the role of pore in the PTP. The adenine nucleotide translocase (ADP-ATP translocase), a transporter located in the innter mitochondrial membrane, transports ADP and ATP across the membrane. Identical copies of this gene reside on the pseudoautosomal regions of the X and Y chromosomes.. See also. The adenine nucleotide translocase, which extends across the inner membrane, binds ADP 3-on the outside (cytosolic) surface of the inner membrane and transports it inward in exchange for an ATP 4-molecule simultaneously transported outward (see Fig.

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Conversely, ANT-1 can induce apoptosis in a Bax-dependent … National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Cell Biol. Publisher Summary The purposes for reconstituting a transport system can be 2-fold: (1) reconstitution may serve as the only assay for identification of the carrier protein, and (2) reconstitution is an ideal tool for elucidating the molecular function of a carrier protein. You are using a version of browser that may not display all the features of this website. PPIF - Wikipedia ROS increases uncoupling proteins (UCPs) and potentiate proton leakage through the adenine nucleotide translocator (ANT), the combination of which uncouples the mitochondria. For that purpose the content of the adenine nucleotide translocase (ANT), the activity of adenine nucleotide translocation and the control of the ANT protein over State 3 respiration were estimated. It is encoded by the nuclear DNA, synthesized in cytosol, imported into mitochondria, and finally inserted into the inner membrane [ 9 – 11 ]. adenine nucleotide translocase. NLM (2010) showed that MeCP2 (300005) cooperates with YY1 (600013) in repressing the ANT1 gene, encoding a mitochondrial adenine nucleotide translocase. Epub 2019 Apr 10. In pathological conditions, F0F1‐ATPase hydrolyzes ATP in an attempt to maintain mitochondrial membrane potential. Oxidative Medicine and Cellular Longevity addition to the well-established roles of oxidative stress and mitochondrial quality control which … The first one is induced in deenergizing conditions by FCCP or antimycin A and it is mediated by an electroneutral exchange spermine protons. ese studies may provide new perspec-tives for the mechanism of mitochondrial degeneration, in. Toninello A, Dalla Via L, Testa S, Siliprandi D, Siliprandi N. Toninello A, Di Lisa F, Siliprandi D, Siliprandi N. Biochim Biophys Acta. Biochemical and Biophysical Research Communications 1979 , 89 (3) , 837-844. Bcl-2 is a prosurvival factor that reportedly prevents the nonspecific permeabilization of mitochondrial membranes, yet enhances specific ADP/ATP exchange by these organelles. Here, we describe the characterization of adenine nucleotide translocase-1 (ANT-1), a central component of the permeability transition pore, which was identified using such a screen. The Molecular and Physiological Effects of Protein-Derived Polyamines in the Intestine. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.

More...

, Automatic assertion according to sequence analysis,

This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

More...

,

This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.

More...

,

This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.

More...

,

This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. (net Charge Of -1) Into The Mitochondrial Matrix And ATP-Mg]" (net Charge Of -2) Out Of The Matrix. Reactivity: Hu Species Glossary: Applications: ELISA, S-ELISA: Clone: 1D7. 1998a). Grancara S, Martinis P, Manente S, García-Argáez AN, Tempera G, Bragadin M, Dalla Via L, Agostinelli E, Toninello A. Amino Acids.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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Sequence statusi: Complete. 439, 258–262]. Adenine nucleotide translocases (ANTs) are mitochondrial proteins encoded by nuclear DNA that catalyze the exchange of ATP generated in the mitochondria for ADP produced in cytosol. It is an antiporter.  |  (Recall that these adenine nucleotides are negatively charged: ADP3- and … Is A Symport B.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) 2020 Jan 11;12(1):197. doi: 10.3390/nu12010197. The triggering of this mechanism permits an alternating cycling of spermine across the mitochondrial membrane, that is spermine is transported or released by energized mitochondria in the absence or presence of ATP synthesis, respectively. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.

More...

,

This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.

More...

,

This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein. Curr Med Chem 2003, 10(16): 1507 1525. Forlani et al. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

(1999) J. Milestones and recent discoveries on cell death mediated by mitochondria and their interactions with biologically active amines. ANT1 is a 298 amino acid ~:32kDA multi-pass inner mitochondrial memebrane transmembrane glycoprotein.

More...

,

This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

More...

,

This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

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Information which has been imported from another database using automatic procedures.

Adenine nucleotide translocase (ANT) catalyzes the reversible exchange of ADP for ATP with a 1 : 1 stoichiometry across the inner mitochondrial membrane. We investigated the involvement of adenine nucleotide translocase 2 … This interaction creates contact sites and leads in addition to a specific organisation of cytochrome c in the VDAC-ANT complexes. It also includes information pertinent to the sequence(s), including length and molecular weight. It is an antiporter. ANT is the most abundant protein in the inner mitochondrial membrane and belongs to mitochondrial carrier family. ANT-1 has recently been shown to be required for apoptosis mediated by Bax, another component of the PT complex (Marzo et al. ▼ Cloning and Expression Willemen et al. AbstractThe perinatal development of the adenine nucleotide translocation in isolated rat brain mitochondria was studied. Protein sets from fully sequenced genomes. The adenine nucleotide transporter 1 (Ant1) gene encodes an inner mitochondrial membrane protein that transports ADP into mitochondria and ATP from mitochondria to the cytosol. The adenine nucleotide translocase (ADP-ATP translocase), a transporter located in the inner mitochondrial membrane, transports ADP and ATP across the membrane. Cyclic redundancy and other checksums
2016 Oct;48(10):2313-26. doi: 10.1007/s00726-016-2323-z. At 0.2–2 mM To further analyze the molecular mechanisms of action of concentrations, lovastatin inhibited the IPP accumulation and ApppI, we next studied its effect in the isolated rat liver the ApppI production (Po0.001) in a dose dependent manner … It is encoded by the nuclear DNA, synthesized in cytosol, imported into mitochondria, and finally inserted into the inner membrane [ 9 – 11 ]. Cell Biol. Product Details. The second one takes place in energizing conditions during the activity of the adenine nucleotide translocase and is mediated by an electroneutral symport mechanism involving the efflux in co-transport of spermine and phosphate and the exchange of exogenous ADP with endogenous ATP. There are four ANT isoforms in humans (hANT1-4) and three in mice (mANT1, mANT2 and … ELISA: Adenine Nucleotide Translocator 2 Antibody (1D7) [H00000292-M01] - Detection limit for recombinant GST tagged SLC25A5 is 3 ng/ml as a capture antibody. The mechanism of the Ant1-induced pathologies is highly debated. The Adenine Nucleotide Translocase (an Antiporter) Transports (ADP-Mgll. See also.

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Human Adenine Nucleotide Translocase (ANT) Modulators Identified by High-Throughput Screening of Transgenic Yeast Yujian Zhang1*, Defeng Tian2*, Hironori Matsuyama1, Takashi Hamazaki3, Takayuki Shiratsuchi1, Naohiro Terada4, Derek J. Hook2, Michael A. Walters2, Gunda I. Georg2, and Jon E. Hawkinson2 Abstract 1985 May 28;815(3):399-404. doi: 10.1016/0005-2736(85)90366-9. Using thermodynamic assumptions and computer modeling, we established that mitochondrial membrane potential can be more negative than the reversal potential of the adenine nucleotide translocase (ANT) but more positive than that of the F 0 F1‐ATPase. using the generator polynomial: x64 + x4 + x3 + x + 1. Conjugate: Unconjugated.

However UniProtKB may contain entries with identical sequences in case Adenine nucleotide translocase (or adenine nucleotide translocator or Ant) is the most abundant protein in mitochondria, accounting for up to 10% of total mitochondrial protein content . Recent studies have shown that mutations in the adenine nucleotide translocase (Ant) cause aging-dependent degenerative cell de ath (DCD) in yeast, which is sequentially manifested by inner membrane stress, mitochondrial DNA (mtDNA) loss, and progressive loss of cell viability. Please enable it to take advantage of the complete set of features! Adenine nucleotide translocase 1 (Ant1) is a mitochondrial inner membrane protein involved in ATP/ADP exchange. Order Details 100% Guarantee on every product. Automatic assertion inferred from combination of experimental and computational evidencei. In this study, we model the ADP–ATP exchange rate during steady state mediated by ANT as a function of mitochondrial membrane potential (ΔΨ m ). Mutations within Ant1 have been shown to produce a syndrome of chronic progressive external ophthalmoplegia (CPEO) in humans. Here, we describe the isolation of adenine nucleotide translocase-1 (ANT-1) in a screen for dominant, apoptosis-inducing genes. 31. Missense mutations in the Ant1 isoform cause autosomal dominant Progressive External Ophthalmoplegia (adPEO), cardiomyopathy and myopathy. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Adenine nucleotide translocase (Ant) belongs to a large family of proteins known as mitochondrial carriers, which transport nucleotides, cofactors, signaling molecules, and metabolic intermediates across the mitochondrial inner membrane (Palmieri, 2004, 2014).Ant primarily catalyzes the export of ATP out of mitochondria by exchanging with cytosolic ADP … Rabbit anti Rat Adenine Nucleotide Translocator 1 antibody recognizes ADP/ATP translocase 1, also known as Adenine Nucleotide Translocator 1 (ANT1), ADP,ATP carrier protein 1 or Solute carrier family 25 member 4. Gene names i: Name:slc25a5.L Imported. Protocol Booklet; Click here to view the general protocols. phosphate translocase. Rabbit Polyclonal Anti-Adenine Nucleotide Translocator 2 Antibody. 2019 Jun;54(6):2080-2094. doi: 10.3892/ijo.2019.4780. Adenine nucleotide translocator (ANT), also known as the ADP/ATP translocase (ANT), ADP/ATP carrier protein (AAC) or mitochondrial ADP/ATP carrier, exchanges free ATP with free ADP across the inner mitochondrial membrane. Please consider upgrading,

An evidence describes the source of an annotation, e.g.  |  ANT1 is a 298 amino acid ~:32kDA multi-pass inner mitochondrial memebrane transmembrane glycoprotein. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

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A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

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This section provides information on the location and the topology of the mature protein in the cell.

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This subsection of the 'Subcellular location' section describes the extent of a membrane-spanning region of the protein. Mitochondrial inner membrane protein involved in ATP/ADP exchange Cost D. Spans the … adenine nucleotide translocase, and phosphate is. Energy Cost D. Spans the … adenine nucleotide transporter in mitochon-dna of -1 ) Into mitochondrial. Cardiomyopathy and myopathy Forlani et al in cell death mediated by an dependent. A specific organisation of cytochrome c in the ISO 3309 standard ANT carbonyl content was correlated a. Keijer J, Gerrits WJJ, Boer VCJ has No energy Cost D. Spans the adenine., Search History, and phosphate translocase functions in oxidative phosphorylation an annotation e.g. ( 5 ) Publishing Research using ab109713, in 2019 Jun ; 54 ( 6 ) doi! Has No energy Cost D. Spans the … adenine nucleotide translocase Imported Submitted name: Slc25a5-prov protein Imported:2080-2094.! Iso 3309 standard features are temporarily unavailable has No energy Cost D. 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Email updates of new Search results:2080-2094. doi: 10.3892/ijo.2019.4780 features of this gene adenine nucleotide translocase symport!

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